OUR TECHNOLOGY
A benchtop system combining digital microfluidics, cell-free protein synthesis, and robust data analytics
At Nuclera, we know that proteins are at the center of life science innovation. Every insight, structure, assay, and screen begins with the right protein.
Getting proteins shouldn’t be a gamble
Today’s protein production workflows put you through cycles of trial and error, where teams lose weeks, money, and resources before they know if the protein is soluble, stable, or active.
What if there was a better path to your protein?
We make this easy by combining unique cell-free technologies and novel digital microfluidics for multiplex protein screening, turning what could take months into a quick, Pipette and Forget™ workflow that gives you your protein in days.
Speed up target identification
In 24 hours, parallel screens rank constructs, solubility tags, expression conditions, and purifiability to find the winners for scale up.
Assay-ready protein in 48 hours
Scale up optimized expression and purification conditions to μg–mg output for structural and functional studies.
Explore multiple protein variations
Screen protein isoforms, mutations, and more for simplified target identification.
Step forward with confidence
Know which proteins are most likely to succeed from day one.
eProtein Discovery™ : How the multiplex protein screening system works
Cell-free Protein Synthesis
Cell-free Protein Synthesis
Express proteins in vitro without cells to bypass cellular constraints and move fast. Each reaction combines your DNA construct with purified cellular translational machinery. Run 8 cell-free expression conditions per screen and tune each with up to two additives (e.g., nanodiscs, detergents, chaperones, cofactors, redox) to improve folding, stability, solubility, and function, even for challenging membrane proteins.
Take high throughput bioreactors, liquid handlers and purification columns out of the equation. Identify optimal expression conditions in a day with our cell-free protein synthesis solution!
Digital microfluidics
Digital microfluidics
eProtein Discovery operates with a digital microfluidics lab-on-a-cartridge device designed to allow droplets of DNA to split, mix, and move within the cartridge, enabling many experiments in the same footprint.
The cartridge automates expression and on-cartridge purification steps so you can test constructs, tags, and conditions in parallel—producing hundreds of datapoints in 24 hours with minimal hands-on time. Pipette and Forget!
On-cartridge characterization
On-cartridge characterization
To aid in protein quantification, DNA constructs include a small peptide detection tag on the C-terminus. Upon binding with a detector protein, the fluorescence-based assay reports only full-length, stable protein. Integrated optics quantify expression and purifiability directly on-cartridge and generate ranked construct/condition pairs. This drives confident selection and increases your chance for success before you scale.
Decision-grade data in the cloud
Decision-grade data in the cloud
The eProtein Discovery Cloud Software delivers exportable reports and contains advanced data analytics covering solubility, expression level, purifiability, and yield. Access all the data to review ranked heatmaps, pick winners, and plan µg–mg scale-up—on site or remotely.
Proteins start here
Move from design to decision in 24 hours. Then scale the best construct/condition pairs overnight to obtain assay-ready protein in another 24 hours. The eProtein Discovery system replaces trial and error with automated, programmable, and purpose-built multiplex screening.
