Resource Library

Part of the in-the-lab instructional video series

Part of the in-the-lab instructional video series

Part of the in-the-lab instructional video series

Part of the in the lab instructional video series

In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours.

An overview of solubility tags and their effects on soluble protein expression, as well as the impact of tag removal, and how it is a necessary and a high-value capability.

This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function.

In this Application Note, we leverage the eProtein Discovery™ system to screen 192 expression conditions and optimize the production of soluble CD19 protein within 24 hours. We successfully expressed and purified three CD19 length variants: full-length CD19, the N-terminal extracellular domain, and the C-terminal intracellular domain, overcoming challenges associated with “difficult-to-express” proteins.

The integration of AlphaFold AI within the eProtein Discovery™ framework brings forth a multitude of advantages, facilitating structure guided protein construct design with unprecedented precision.