Resource Library

Unlock GPCRs and other challenging membrane proteins with a cell-free expression workflow that delivers cryo-EM–ready complexes and connects in vitro structures to in vivo function.

Join Cube Biotech and Nuclera to discover a rapid, scalable workflow for expressing and stabilizing complex membrane proteins. Learn how Cube Biotech’s membrane mimetics combined with Nuclera’s eProtein Discovery system enable stable, functional, assay-ready membrane proteins in just 48 hours. This webinar highlights case studies on key drug targets, demonstrating how optimized membrane protein production…

In this webinar, Dr. Manoj Saxena (Birkbeck, University of London) presents a cell-free, microfluidic multiplex screening workflow that rapidly optimizes soluble transcription factor production. Using this approach, a novel target, Transcription Factor L (TF L) with LIM domains, a homeodomain, and extensive disorder, was produced alongside domain constructs and functionally validated by DNA-binding assays within 48 hours. This workflow moves researchers from disorder to data, enabling assay-ready proteins and accelerating discovery in developmental biology, regenerative medicine, and therapeutic development.

Join experts from IDT, Nuclera, and Aldevron for a webinar showcasing a fully integrated solution that takes you from gene to assay-ready protein in just days — not weeks.

This webinar introduces the latest update to eProtein Discovery™, allowing researchers to customize cell-free expression conditions to improve yields of co-factor dependent membrane proteins.

Screen, Scale, Capture, Characterize – DNA to Protein Characterization in One Week Understanding drug–target interactions is essential to improve leads, optimize pharmacokinetics and predict in vivo efficacy. The process of generating soluble, functional drug targets and studying their interactions with drugs can be complicated and time-consuming, with multiple repeats needed to optimize protein expression and…

In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours.

The integration of AlphaFold AI within the eProtein Discovery™ framework brings forth a multitude of advantages, facilitating structure guided protein construct design with unprecedented precision.

The eProtein Discovery™ System can screen multiple protein expression and purification profiles and deliver reliable proteins in-hand in less than 48 hours. Revolutionizing laboratory workflows and making a demonstrable impact in terms of time to publish in academia and drug discovery timelines.

Overcoming the Inexpressible: How cell-free protein synthesis solved the E. coli toxicity hurdle. Learn how the eProtein Discovery™ enabled optimization of the expression conditions for an GCN5-Related N-Acetyltransferase protein and gain insights into its antibiotic resistance mechanism.