The Smart Cartridge - Enabling Pipette and ForgetTM within eProtein DiscoveryTM

Pipette reagents into the Smart Cartridge to get comprehensive data on protein solubility, purifiability and purifiable yield in a day!


Features and Benefits

Powered by digital microfluidic technology, software controlled digital signals guide the movement of droplets on the Smart Cartridge surface to enable splitting, dispensing and merging of biological reagents. Pipette DNA, cell free expression reagents and purification solutions on the Smart Cartridge and the technology will deliver the rest.

Gain precise control of your eGene™ constructs and reagents to screen and discover optimal expressing conditions within 24 hours, accelerating target selection. A simple set-up allows anyone to run the system with minimal training.


Microfluidic lab-on-a-cartridge

Automate separate workflows of expression, characterization and purification all onto one cartridge


Pipette and forgetTM

Pipette reagents for screen and purification and eProtein DiscoveryTM delivers the rest


192X Multiplex expression optimization

192X screening pairs analyzed in one run and then obtainable candidates selected for purification

Examples of proteins synthesized with eProtein Discovery™


Protein Class

Cytokines/Growth Factors
Protein Name
  • Interferon gamma (IFN-γ)
  • Programmed cell death protein 1 (PD1)
  • Erythropoietin
  • Vascular endothelial growth factor (VEGF)
  • Interleukin-2 (IL-2)
Transcription Factors
Other Enzymes

Technical Specifications

Multiplex optimization 24 DNA constructs x 8 conditions = 192 data points screened in parallel
Fluorescent end point detection capability Fluorescent data for 192 sites on cartridge
Magnetic bead-based purification Miniaturized protein purification on chip for purifiability assessment
High resolution digital droplet control 250k individual pixels on cartridge enables precise droplet acrobatics
Electrowetting on dielectric Smart Cartridge Digital signals to manipulate droplets of diverse chemistry
Protected by vacuum deposition smart coating technology Coating optimized to enable precise movement of small volumes

Featured Resource

Other Components



eProtein Discovery™ instrument orchestrates the rapid protein prototyping workflow.



Ready-to-use eProtein DiscoveryTM reagents for screening and cell-free protein synthesis.



Cloud-based, user-friendly software facilitates the experimental design, screening, and analysis of results.

Frequently Asked Questions

The cartridges are meant for single use only and cannot be reused.

Cartridges are loaded on the instrument drawer. Error proof cartridge loading design enables cartridges to be positioned on the instrument drawer accurately each time.

Reagents can be pipetted into designated ports on the cartridge. Clearly labeled ports and intuitive software guides the user to dispense reagents into the appropriate ports. Pipette and Forget™!

Still have questions?