The Smart Cartridge - Enabling Pipette and ForgetTM within eProtein DiscoveryTM
Pipette reagents into the Smart Cartridge to get comprehensive data on protein solubility, purifiability and purifiable yield in a day!
Features and Benefits
Powered by digital microfluidic technology, software controlled digital signals guide the movement of droplets on the Smart Cartridge surface to enable splitting, dispensing and merging of biological reagents. Pipette DNA, cell free expression reagents and purification solutions on the Smart Cartridge and the technology will deliver the rest.
Gain precise control of your eGene™ constructs and reagents to screen and discover optimal expressing conditions within 24 hours, accelerating target selection. A simple set-up allows anyone to run the system with minimal training.
Automate separate workflows of expression, characterization and purification all onto one cartridge
Pipette and forgetTM
Pipette reagents for screen and purification and eProtein DiscoveryTM delivers the rest
192X Multiplex expression optimization
192X screening pairs analyzed in one run and then obtainable candidates selected for purification
Examples of proteins synthesized with eProtein Discovery™
- Interferon gamma (IFN-γ)
- Programmed cell death protein 1 (PD1)
- Vascular endothelial growth factor (VEGF)
- Interleukin-2 (IL-2)
|24 DNA constructs x 8 conditions = 192 data points screened in parallel
|Fluorescent end point detection capability
|Fluorescent data for 192 sites on cartridge
|Magnetic bead-based purification
|Miniaturized protein purification on chip for purifiability assessment
|High resolution digital droplet control
|250k individual pixels on cartridge enables precise droplet acrobatics
|Electrowetting on dielectric Smart Cartridge
|Digital signals to manipulate droplets of diverse chemistry
|Protected by vacuum deposition smart coating technology
|Coating optimized to enable precise movement of small volumes
Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression
Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.
Application Note: Rapid cell-free expression and solubility screening to obtain active human VEGF with eProtein Discovery™ platform
VEGF is a key regulator of angiogenesis and plays a central role in the process of tumor growth, making it an appealing target for anticancer therapeutics. This platform demonstrates time-saving for both soluble construct identification and micrograms of active proteins obtained in 48 hours.
Frequently Asked Questions
The cartridges are meant for single use only and cannot be reused.
Cartridges are loaded on the instrument drawer. Error proof cartridge loading design enables cartridges to be positioned on the instrument drawer accurately each time.
Reagents can be pipetted into designated ports on the cartridge. Clearly labeled ports and intuitive software guides the user to dispense reagents into the appropriate ports. Pipette and Forget™!