Reagents to power eProtein DiscoveryTM
Specialized reagents that drive your entire protein workflow —supporting DNA construct preparation, cell-free expression and purification screening, and seamless off-cartridge scale-up with accurate yield quantification. Assay-ready protein in 48 hours.
Features and benefits
The eProtein Discovery™ reagents enable a complete, multiplex screening workflow, from construct design to assay-ready protein, in 48 hours.
Simplify construct preparation with ready-to-use linear or circular DNA. Screen DNA constructs on the eProtein Discovery system using the Soluble or Membrane multiplex screening workflows against a core cell-free expression reagent customized with a panel of additives to optimize folding, stability, solubility, and function. Integrated detection, purification steps, and optional solubility tags surface the best construct/condition pairs for rapid, streamlined scale-up.
With fewer manual steps and faster turnaround, you can confidently progress to downstream applications.
Flexible DNA formats
Start rapidly with ready-to-use linear or circular DNA without the need to prepare DNA yourself.
Cell-free Core reagent & additives
Customizable cell-free transcription and translation machinery to produce soluble and membrane proteins
Scale-up reagents
Scale protein production in cell-free reagents, guided by your protein optimization screen.
eProtein Quantification kit
Highly specific fluorescence-based quantification system for measuring protein yield post-scale-up.
eProtein Discovery produces proteins of diverse origin
Human soluble proteins synthesized with eProtein Discovery
Protein Class
Protein Name
- ScFv
- VHH
Challenging membrane proteins synthesized with eProtein Discovery
Membrane Protein Class
Technical specifications
| Custom Linear eGene Constructs | Ready-to-use and validated linear DNA constructs ready to pipette onto an eProtein Discovery cartridge |
| Circular eGene Constructs | Ready-to-use, sequence-verified circular DNA constructs delivered as complete expression cassettes. Compatible with both cell-free and E. coli scaling. |
| Linear eGene Prep Kit | Convert gene fragments into linear DNA expression cassettes with C-terminal detection and purification tags (FlexiVariant™ Screen) and optional N-terminal solubility tags (Solubility Tag Screen). Ideal for scientists who wish to prepare DNA on their own. |
| Circular eGene Prep Kit | Enzymatic preparation kit for circular eGene™ (plasmid) constructs. No cloning or PCR required. 30 min protocol. |
| Cartridge Reagent Kit | Reagents for expression and purification screening of soluble proteins on the eProtein Discovery system. |
| Membrane Cartridge Reagent Kit | Reagents for expression and purification screening for membrane proteins on the eProtein Discovery system. |
| Scale-up Kit | Allows seamless in-tube production of the target protein using optimal conditions from screening. |
| eProtein Quantification Kit | Leverages the same assay used on-cartridge to deliver accurate yield metrics from in-tube cell-free or E. coli scale-up. |
| Cell-free Core reagent | Cell-free expression reagent, customizable with Nuclera-supplied or custom chosen additives. |
| Additive: GSSG | Produces an oxidizing environment to promote disulfide bond formation. |
| Additive: PDI/GSSG | Oxidizing conditions with protein disulfide isomerase to promote correct disulfide bond formation. |
| Additive: Metal ions (ZnCl₂, CaCl₂, or MnCl₂) | Supports metalloprotein folding and catalytic activity. |
| Additive: Cofactor mix (NAD, Acetyl-CoA, FAD, SAM, and PLP) | Promotes protein folding, stabilization, and functional activity. |
| Additive: TRXB1 | Chaperone that binds to unfolded or misfolded proteins, preventing aggregation, and assisting in their refolding or degradation. |
| Additive: DnaK | Chaperone that binds to unfolded or misfolded proteins, preventing aggregation, and assisting in their refolding or degradation. |
| Additive: 3C Protease | Enables in situ solubility tag cleavage |
| Custom additives | Integrate your own additives into our protein optimization tool, tailored towards the structure and function of your protein. |
Approved Circular eGene suppliers
Featured Resources
Flyer: eProtein Quantification Kit Flyer
Achieve accurate, consistent protein quantification at every scale. The same fluorescence-based on-cartridge assay used during screening is now available for scale-up, enabling seamless transition without revalidation or method transfer. With superior sensitivity and specificity compared to absorbance or SDS-PAGE, the eProtein Quantification Kit delivers precise, reproducible results across your workflow, and is fully compatible with…
Brochure: Circular eGene™ and Linear eGene™ Comparison
Discover how Nuclera’s eProtein Discovery™ system gives you the freedom to choose between linear and circular eGene™ constructs. Whether you need PCR-based, cost-effective screening with linear eGene™, or ready-to-use, sequence-verified circular eGene™ plasmids for seamless expression in cell-free and E. coli. Compare formats, workflows, and timelines to find the best fit for your protein discovery needs.
