APPLICATIONS
Accelerate target identification and validation with eProtein Discovery™
Increase your chances of successful drug discovery with multiplex protein screening that provides decision-grade data in 24 hours and assay-ready protein in 48 hours.
Know your best targets from day one
Proteins are at the starting point in the drug discovery process and every scientist involved will need rapid access to high-quality, novel proteins. The problem: identifying and obtaining winning proteins requires tremendous resources, a significant workforce, and a touch of luck.
eProtein DiscoveryTM uses unique technology to empower protein scientists and protein engineers to investigate a wide range of candidate proteins, variations, and conditions simultaneously, in a multiplexed fashion.
Optimized to reliably handle screening for protein expression, characterization, and purification, our system ensures optimal drug target identification and validation directly from your benchtop in record time.
Fit for tough classes
Suitable for challenging proteins including transcription factors, E3 ligases, and membrane proteins (GPCRs, transporters, ion channels, and enzymes).
A dedicated workflow for membrane proteins
Screens stabilizers (nanodiscs, detergents, cofactors, redox, and chaperones) to find what works.
De-risk targets early
Rank constructs, tags, and expression conditions in a single 24-hour run to identify the best hits.
Get usable material fast
Overnight scale-up yields µg–mg for structural and functional assays with less than 2 hours of hands-on time.
Revolutionize target identification with eProtein DiscoveryTM
Screen proteins faster
Screen proteins faster
Protein expression programs are lengthy processes, requiring an average of 4-6 weeks to complete and significant investment which can vary based on the protein.
Nuclera’s cartridge offers the possibility to test multiple DNA constructs in 8 different cell-free expression systems simultaneously. The soluble protein workflow tests 24 DNA constructs, giving a total of 192 expression datapoints and 30 purification datapoints, while the membrane protein workflow tests 11 DNA constructs to generate 88 expression and 88 purification datapoints.
On-cartridge fluorescence quantifies full-length, stable protein, so you can advance with confidence from day one. This significantly reduces the turnaround time of target discovery because our technology identifies the most promising proteins from day one.
Get soluble proteins
Overcome protein solubility challenges
Protein solubility is fundamental in drug development and pharmaceutical applications. Use customizable Cell-free Blends (e.g., chaperones, redox pairs, metal ions, cofactors) plus solubility tags and construct variants to improve folding and solubility. In 24 hours, ranked reports show which construct/condition pairs yield soluble, purifiable protein; the next day (or overnight), scale to µg–mg of assay-ready material.
With eProtein Discovery, you can confidently move forward knowing that you have the ideal, soluble protein in your hands
Get active proteins
Get functionality from the start
Not all proteins synthesized from DNA constructs will be biologically active. Therefore, it is essential to test and validate protein functionality early on to ensure the viability of future therapeutic use.
Our rapid eProtein Discovery system offers insights regarding specific traits and functionality of your proteins within 24 hours, increasing your chances of identifying the most suitable candidate fit for your purpose while minimizing your investment and decision-making time.
Get purifiable proteins
Reduce downstream purification rework
Access valuable information right after your eProtein Discovery -assisted screen! Our eProtein Discovery Cloud Software provides decision-grade data on protein expression, purifiability, and purified yield before you commit resources.
Use the ranked results to scale the best pairs to µg–mg for structural and functional studies directly on your benchtop.
How does eProtein Discovery fit into your workflow?
Employ eProtein Discovery at the drug discovery phase for target identification and validation. eProtein Discovery allows protein scientists and engineers to map the path to soluble, active protein from day one without trial and error.
