AI-powered protein design for enhanced solubility
Protein folding, solubility and functional stability remain key obstacles in protein engineering, structural biology, and early-stage target discovery. Traditional variant design methods are slow, iterative, and often yield low-expression or misfolded proteins.
AlphaFold integration into eProtein Discovery™ transforms protein design into a smart, structure-guided workflow. Instantly visualize 3D structures, identify key regions for truncation or mutation, and screen variants for soluble expression, all in one automated system. Move from protein predictions to folded, active protein in just 48 hours.
With AlphaFold-guided protein variant design and eProtein Discovery screening, protein variant design is no longer a guessing game, it’s a data-driven strategy for success.
Structural visualization
AlphaFold2 structural predictions with model confidence, hydrophobicity, and domain mapping
Protein feature annotation
Identify functional domains, motifs, and key regions
Bioinformatic insights
Bioinformatics tools: MSA, pLDDT, PAE for in-depth structural assessment
Protein variant design
Engineer stable, functional variants guided by protein feature functional annotations for targeted truncation/mutation
AlphaFold2 integration into eProtein Discovery
AI-powered structural prediction
Three powerful views. Smarter protein design.
Protein folding and solubility is a major challenge in structural biology and biopharmaceuticals, limiting yield, functionality, and purification efficiency. Traditional screening methods rely on labor-intensive, trial-and-error approaches that slow progress.
Nuclera’s system offers three dynamic visualization modes: model confidence, feature and domain mapping, and residue hydrophobicity. Gain deeper insights into protein structure, zoom into residues, analyze intramolecular bonds, and refine designs with precision.
AlphaFold-powered visualizations help you identify problematic regions and preserve functionally critical domains. Make informed design decisions based on predicted confidence and hydrophobicity profiles.
Structural predictions of matrix metalloproteinase 1 (MMP-1) in three visualization modes: model confidence, feature and domain mapping, and residue hydrophobicity.
Protein variant generation
Design protein variants with confidence
AlphaFold structural predictions within eProtein Discovery are enriched with functional domain annotations, key residues, disordered regions, and transmembrane segments.
Easily identify critical regions for truncation or mutation, removing aggregation-prone domains while preserving essential structural and functional elements, maximizing your chances of obtaining soluble, folded, active protein.
Beyond construct design, these structural insights enable smarter customization of cell-free expression conditions, taking into account stabilizing features such as disulfide bonds, co-factor binding sites, and active domains.
Identifying key functional domains enables smarter protein variant design.
Bioinformatic insights
Advanced bioinformatics for structural evaluation
eProtein Discovery™ pairs AlphaFold’s predictive power with advanced bioinformatics tools to validate and refine structural insights.
- Multiple Sequence Alignment (MSA): Uncover conserved regions and evolutionary signals across species to inform variant stability.
- Predicted Local Distance Difference Test (pLDDT): Assess the reliability of structural predictions at the residue level.
- Predicted Aligned Error (PAE): Evaluate confidence in the relative positioning of residue pairs within the predicted structure.
Bioinformatics tools: MSA, pLDDT, PAE for in-depth structural assessment.
Maximize soluble protein production
From Al designed to eProtein Discovery made
Following protein variant design, eProtein Discovery leverages digital microfluidics for nanodroplet screening, optimizing each construct across customizable cell-free expression conditions tailored to structural predictions.
The protein variant and expression conditions that deliver optimal protein folding, solubility, and yield are seamlessly scaled up to µg–mg quantities, providing assay-ready protein in just 48 hours.
This all-in-one system accelerates protein variant evaluation, streamlines scale-up, and speeds up protein availability for research and drug discovery.
From prediction to soluble protein in 48 hours powered by eProtein Discovery.
From sequence to soluble protein in 48 hours
Rapidly express and purify soluble, properly folded proteins optimized through structure-guided design. Go from hypothesis to protein with a seamless, all-in-one system.
The eProtein Discovery™ Soluble Protein Workflow with AlphaFold integration.
What can the eProtein Discovery produce?
eProtein Discovery expresses proteins from a wide range of organisms, including human, animal, plant, bacterial, fungal, yeast, and viral sources. It supports diverse protein classes and sizes, from small 18 kDa proteins to large 300 kDa complexes. As our technology evolves, so does its capability to express even more protein types, expanding the possibilities for discovery across research and therapeutic applications.
