On-Demand Webinar – Overcoming the Inexpressible: How cell-free protein synthesis solved the E. coli toxicity hurdle

October 23, 2023

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Introduction:

In this webinar Prof. Joleen Masschelein from VIB/ KU Leuven in Belgium, shares their experience using the eProtein Discovery platform to produce a difficult-to-express protein that has evaded all other attempts at expression

The eProtein Discovery enabled the team to optimize the expression conditions for an GCN5-Related N-Acetyltransferase protein and gain insights into its antibiotic resistance mechanism. The platform provides an efficient and streamlined approach to protein expression, characterization, and purification.

About eProtein Discovery:

Nuclera’s eProtein Discovery platform combines digital microfluidic droplet automation with cell-free protein synthesis technologies, it empowers protein scientists to identify the best conditions for expressing and purifying proteins of interest within 24 hours, all on a single consumable cartridge.

The system offers a significant advantage over traditional protein expression methods, allowing researchers to save time and resources by simplifying and automating the process. Its ability to handle multiple genes and customizable cell-free blends makes it a valuable tool for protein scientists in academia and the biopharma industry.

About The Masschelein Lab, VIB-KU Leuven:

We study and engineer host-associated bacteria and their specialized metabolites for in vivo therapeutic and agrochemical applications. We are particularly interested in discovering new bioactive natural products from host-associated bacteria elucidating unusual pathways for natural product biosynthesis unravelling the ecological role and mode of action of natural products microbiome engineering for the production of high-value natural products and other therapeutic applications

Our work is multidisciplinary, combining molecular microbiology, synthetic biology, structural biology, metabolomics, enzymology, bioinformatics, analytical chemistry and chemical synthesis.

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