APPLICATIONS
Optimized assay-ready membrane protein in 48 hours with eProtein Discovery™ multiplex screening
Our customizable membrane stabilizing screening solution delivers purified protein – optimized for folding, stability, yield, and function – in 48 hours.
Screen your way to stable membrane proteins
Membrane proteins account for 60% of drug targets. Yet, producing these highly challenging proteins using traditional methods often enter a cycle of trial-and-error failures resulting in misfolded or aggregated protein and, ultimately, low yields.
The eProtein Discovery™ System combines customizable cell-free expression with digital microfluidics to deliver multiplex protein screening to identify the membrane protein variant and stabilization/expression conditions with the highest chance of success, so you know where to start.
Explore your path to stable, purified, active, assay-ready membrane protein today.
Multiplexed membrane protein screening
Screen 11 membrane proteins in 8 customizable, stabilizing cell-free expression conditions in 24 hours. Identify the best nanodiscs, detergents, chaperones, cofactors, and redox conditions to optimize stability and yield.
Rapid detection & characterization
On-cartridge fluorescence assay quantifies only full-length, stable membrane proteins, providing decision-grade data from 88 expression and purification datapoints to guide selection.
Assay-ready protein
Scale the top construct/condition pairs off-cartridge overnight to µg–mg of purified protein, ready for structural and functional assays, in 48 hours.
Unlock challenging membrane proteins
Whether you work with human, plant, or bacterial targets—GPCRs, ion channels, transporters, or membrane enzymes—eProtein Discovery identifies the conditions that fit your protein.
The eProtein Discovery membrane protein workflow
With eProtein Discovery, multiplex membrane protein screening and protein production is faster than ever. Design constructs, screen with customizable, membrane-stabilizing cell-free conditions using automated digital microfluidics, and scale up stable, active membrane proteins for any downstream application.
eProtein Discovery: Your path to membrane protein
Optimize membrane protein production
Identify the best constructs and conditions with multiplex protein screening
Nuclera’s multiplex protein screening system accelerates membrane protein production. Within 24 hours, screen constructs against fine-tuned, customizable expression environments containing membrane stabilizing additives, such as nanodiscs, detergents and co-factors. Tailor folding and stability parameters to reduce misfolding and accelerate downstream workflows.
From GPCRs and ion channels to transporters and enzymes, this system delivers optimized conditions for high-yield, purified, assay-ready membrane proteins.
Automated & miniaturized
Increase screening throughput with Pipette and Forget™ workflow
Automated digital microfluidics enables efficient, hands-free workflows. The nanodroplet screening format reduces reagent consumption, saves time, and minimizes experimental variability while increasing throughput.
The built-in, on-cartridge fluorescence assay ensures only full-length, stable proteins are quantified. Advanced imaging algorithms track nanodroplet behavior—detecting subtle fluorescence patterns, identifying aggregation zones, and distinguishing soluble fractions with high precision. This enables accurate, real-time QC and confident decision-making.
Stable & purified membrane proteins
From DNA to purified, stable membrane protein in 48 hours
Scale up expression and purification seamlessly using data from your screen to produce purified, assay-ready membrane protein (µg–mg quantities). Easily move from construct design to functional protein, without aggregation, precipitation, or iterative troubleshooting.
Need more protein? Simply scale up reagents proportionally to increase yield. Yield predictions obtained from the digital microfluidics screen align with scale-up results.
Reduce iterative optimization cycles and accelerate the path to structural insights and functional validation.
Assay-ready protein
Functional, cryo-EM ready membrane proteins
Proteins are at the center of life science innovation, but you can’t begin experiments without them. The real excitement starts after you have those proteins in hand. Scale up the best conditions to obtain stable, functional membrane proteins within 48 hours, ready for cryo-EM, activity assays, and rapid target validation without months of trial and error.
This transformative system accelerates early-stage target discovery and drug development, enabling confident protein identification, validation, and optimization with minimal hands-on time.
Explore every membrane protein class
From GPCRs and ion channels to transporters and enzymes, here are examples of membrane proteins produced using the eProtein Discovery System. Ready to tackle challenging membrane proteins? Start with eProtein Discovery!
Protein Class
What can the eProtein Discovery system produce?
Multiplex screening with eProtein Discovery expresses proteins from a wide range of sources—including human, animal, plant, bacteria, fungi, yeast, and virus. It supports diverse protein classes and sizes, including GPCRs, ion channels, transporters, enzymes, cytokines/growth factors, transcription factors, and more. As the technology continues to evolve, so does its ability to express even more protein types, expanding opportunities for discovery across both research and therapeutic applications.
