AI-powered protein design for enhanced solubility
The best protein is one that is stable, soluble, and properly folded. However, arriving at this protein with traditional variant design methods can be slow, iterative, and often results in low yield or misfolded proteins.
eProtein Discovery™ transforms protein design into a smart, structure-guided workflow supported by AI tools. Instantly visualize 3D structures, identify key regions for truncation or mutation, and screen variants for soluble expression, all in one automated system. Move from protein predictions to folded, active protein in just 48 hours.
With AlphaFold-guided protein variant design and eProtein Discovery multiplex protein screening, protein variant design is no longer a guessing game. It’s a data-driven strategy for success.
Structural visualization
AlphaFold2 structural predictions with model confidence, hydrophobicity, and domain mapping modes.
Protein feature annotation
Identify functional domains, motifs, and key regions
Validate and refine structural insights
Bioinformatics tools like MSA, pLDDT and PAE enable in-depth structural assessment.
Protein variant design
Engineer stable, functional variants guided by protein feature functional annotations for targeted truncation/mutation
AlphaFold2 integration into eProtein Discovery
AI-powered structural prediction
Three powerful views. Smarter protein design.
Nuclera’s system offers three dynamic visualization modes: model confidence, feature and domain mapping, and residue hydrophobicity. Gain deeper insights into protein structure, zoom into residues, analyze intramolecular bonds, and refine designs with precision.
AlphaFold-powered visualizations help you identify problematic regions and preserve functionally critical domains. Make informed design decisions based on predicted confidence and hydrophobicity profiles.
Protein variant generation
Design protein variants with confidence
AlphaFold structural predictions within eProtein Discovery are enriched with annotations of functional domains, key residues, disordered regions, and transmembrane segments.
Easily identify critical regions for truncation or mutation, removing aggregation-prone domains while preserving essential structural and functional elements – maximizing your chances of obtaining soluble, folded, active protein.
Beyond construct design, these structural insights enable smarter customization of cell-free expression conditions, taking into account stabilizing features such as disulfide bonds, co-factor binding sites, and active domains.
Bioinformatic insights
Advanced bioinformatics for structural evaluation
eProtein Discovery pairs AlphaFold’s predictive power with advanced bioinformatics tools to validate and refine structural insights.
- Multiple Sequence Alignment (MSA): Uncover conserved regions and evolutionary signals across species to inform variant stability.
- Predicted Local Distance Difference Test (pLDDT): Assess the reliability of structural predictions at the residue level.
- Predicted Aligned Error (PAE): Evaluate confidence in the relative positioning of residue pairs within the predicted structure.
Maximize soluble protein production
From Al designed to proteins in hand
Following protein variant design, eProtein Discovery leverages digital microfluidics for nanodroplet screening, optimizing each construct across customizable cell-free expression conditions tailored to structural predictions.
The protein variant and expression conditions that deliver optimal protein folding, solubility, and yield are seamlessly scaled up to µg–mg quantities, providing assay-ready protein in just 48 hours.
This all-in-one system accelerates protein variant evaluation, streamlines scale-up, and speeds up protein availability for research and drug discovery.
From sequence to protein in 48 hours
Rapidly express, screen, and scale soluble, properly folded proteins optimized through structure-guided design. Map your path to your protein in 24 hours and have proteins in hand in 48 hours.
The eProtein Discovery™ Workflow with AlphaFold integration.
What can the eProtein Discovery produce?
eProtein Discovery expresses proteins from a wide range of sources, including human, animal, plant, bacteria, fungi, yeast, and virus. It supports diverse protein classes and sizes, including soluble proteins and membrane proteins. As our technology evolves, so does its capability to express even more protein types, expanding the possibilities for discovery across research and therapeutic applications.
