WEBINAR
Accelerating Transcription Factor Discovery with Cell-Free Systems
Summary:
Highly disordered transcription factors are master regulators of gene expression, yet their structural disorder and key functional domains makes them notoriously hard to express and purify. Conventional hosts (E. coli, insect, mammalian, yeast) often yield insoluble or degraded protein, with trial-and-error approaches hindering structural and functional studies.
In this webinar, Dr. Manoj Saxena (Birkbeck, University of London) presents a cell-free, microfluidic multiplex screening workflow that rapidly optimizes soluble transcription factor production. Using this approach, a novel target, Transcription Factor L (TF L) with LIM domains, a homeodomain, and extensive disorder, was produced alongside domain constructs and functionally validated by DNA-binding assays within 48 hours. This workflow moves researchers from disorder to data, enabling assay-ready proteins and accelerating discovery in developmental biology, regenerative medicine, and therapeutic development.
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Webinar Topics:
- Scientific and technical barriers in producing highly disordered transcription factors, and implications for target validation and therapy.
- Cell-free multiplex screening: rapidly identifying constructs, tags, additives, and blends; generating 192 expression and 30 purification datapoints to optimize yield and solubility.
- A case study on TF L, a novel spaghetti-like transcription factor. Both full-length and domain variants to study structure-function relationships within 48 hours.
Learning outcomes:
- How to adopt cell-free expression and digital microfluidics to screen hundreds of conditions in parallel to maximize soluble yield.
- Understand the strategies used to obtain functional transcription factors in 48 hours.
- How to apply insights from cell-free workflows to accelerate research in developmental biology, regenerative medicine, and therapeutic discovery.
Speaker:
Manoj Saxena PhD
About eProtein Discovery
Nuclera's eProtein Discovery™ system combines digital microfluidic droplet automation with cell-free protein synthesis technologies, it empowers protein scientists to identify the best conditions for expressing and purifying proteins of interest within 24 hours, all on a single consumable cartridge.
The system offers a significant advantage over traditional protein expression methods, allowing researchers to save time and resources by simplifying and automating the process. Its ability to handle multiple genes and customizable cell-free blends makes it a valuable tool for protein scientists in academia and the biopharma industry.
