WEBINAR
Rapidly Express Optimized Membrane Proteins in 48 hours
March 27, 2025 | 8 AM (PDT), 11 AM (EDT), 3 PM (UK)
Introduction:
Did you know that 30% of human proteins require cofactors for stability and function, and over 60% of drug targets are membrane proteins? Despite their importance, these proteins are among the hardest to express, often failing to fold properly or remain stable in traditional systems.
This webinar introduces the latest update to eProtein Discovery™, allowing researchers to customize cell-free expression conditions to improve yields of co-factor dependent membrane proteins. We will explore how custom additives have enabled the successful production of DHODH, a cofactor-dependent enzyme, and MsbA, a membrane protein stabilized in nanodiscs for cryo-EM studies. You will also learn how high-throughput multiplexing can identify optimal conditions in just 24 hours, making difficult-to-express proteins more accessible for research and drug discovery.
Sign up:
Webinar Topics:
- How eProtein Discovery™ improves expression success in challenging proteins, including membrane proteins
- Case studies on DHODH and MsbA, demonstrating protein production optimized for cryo-EM and functional assays
- How eProtein Discovery generates proteins that are ready to use for downstream applications, including structural biology and activity studies
What to expect?
- Best practices for optimizing membrane proteins and cofactor-dependent enzymes for functional validation
- How eProtein Discovery™ accelerates difficult protein workflows, making proteins ready for drug discovery and mechanistic studies
Speaker:
Toby has 19 years experience in product development for disruptive NGS and Epigenetics-tools companies such as Solexa, Illumina and PacBio.
At Nuclera he leads the Product Development Division, developing cutting-edge protein expression, optimization and detection workflows and integrating them into the eProtein Discovery system.
Toby is the tallest member of the Nuclera team and has a love of Marmite and all things Scottish.
About eProtein Discovery
Nuclera's eProtein Discovery™ system combines digital microfluidic droplet automation with cell-free protein synthesis technologies, it empowers protein scientists to identify the best conditions for expressing and purifying proteins of interest within 24 hours, all on a single consumable cartridge.
The system offers a significant advantage over traditional protein expression methods, allowing researchers to save time and resources by simplifying and automating the process. Its ability to handle multiple genes and customizable cell-free blends makes it a valuable tool for protein scientists in academia and the biopharma industry.
