Reagents to power eProtein DiscoveryTM
Specialized protein reagents designed to drive your workflow—supporting DNA construct preparation, expression and purification screening, and seamless off-cartridge scale-up and yield quantification for optimal protein discovery.
Features and Benefits
The protein reagents within the eProtein Discovery™ system power a complete end-to-end workflow, from gene to assay-ready protein, in just 48 hours.
Rapidly prepare DNA constructs using eGene™ Prep Kits, and screen using either our Soluble or Membrane Protein workflows with the cell-free core reagent, tailored using specialized additives. Integrated detection, purification, and optional solubility tags help you optimize protein obtainability, enhance solubility, and streamline scale-up.
With fewer manual steps and faster turnaround, you can confidently progress to downstream applications.
eGene™ Prep Kits
Design and prepare DNA constructs with pre-set fusion tags for detection, purification, and solubility screening.
Cell-free Core Reagents & Additives
Customizable reagents tailored to meet the diverse needs of soluble to membrane proteins.
Scale Up Solutions
Scale protein production in cell-free or E. coli, guided by your protein optimization screen.
eProtein Quantification Kit
Highly specific fluorescence-based quantification system for measuring protein yield post-scale-up.
eProtein Discovery™ produces proteins of diverse origin
Human soluble proteins synthesized with eProtein Discovery™
Protein Class
Protein Name
- ScFv
- VHH
Challenging membrane proteins synthesized with eProtein Discovery™
Protein Class
Technical Specifications
| eGene™ Prep Kit: FlexiVariant™ Screen (for linear eGene™ only) | Convert gene fragments into linear DNA expression cassettes with C-terminal detection and purification tags |
| eGene™ Prep Kit: Solubility Tag Screen (for linear eGene™ only) | Convert gene fragments into linear DNA expression cassettes with additional N-terminal solubility tag options |
| Circular eGene™ Prep Kit | Enzymatic preparation kit for circular eGene™ (plasmid) constructs. No cloning or PCR required. |
| Cartridge Reagent Kit | Includes reagents for expression and purification screening of soluble proteins on the eProtein Discovery™ system. |
| Membrane Cartridge Reagent Kit | Includes reagents for expression and purification screening for membrane proteins on the eProtein Discovery™ system. |
| Scale-up Kit | Allows seamless in-tube production of the target protein using optimal conditions from screening. |
| eProtein™ Quantification Kit | Leverages the same assay as used on-cartridge to deliver accurate yield metrics post in-tube cell-free or E. coli scale up. |
| Cell-free Core reagent | Cell-free expression reagent, customizable with Nuclera-supplied or custom chosen additives. |
| Additive: GSSG | Produces an oxidizing environment to promote disulfide bond formation. |
| Additive: PDI/GSSG | Oxidizing conditions with protein disulfide isomerase to promote correct disulfide bond formation |
| Additive: Metal ions (ZnCl₂, CaCl₂, or MnCl₂) | Supports metalloprotein folding and catalytic activity |
| Additive: Cofactor mix (NAD, Acetyl-CoA, FAD, SAM and PLP) | Promotes protein folding, stabilization, and functional activity. |
| Additive: TRXB1 | Chaperone binds to unfolded or misfolded proteins, preventing aggregation, and assisting in their refolding or degradation |
| Additive: DnaK | Chaperone binds to unfolded or misfolded proteins, preventing aggregation, and assisting in their refolding or degradation |
| Additive: 3C Protease | Enables in situ solubility tag cleavage |
| Custom Additives | Integrate your own additives into our protein optimization tool, tailored towards the structure and function of your protein. |
Approved Circular eGene suppliers
Featured Resources
Flyer: eProtein™ Quantification Kit Flyer
Achieve accurate, consistent protein quantification at every scale. The same fluorescence-based on-cartridge assay used during screening is now available for scale-up, enabling seamless transition without revalidation or method transfer. With superior sensitivity and specificity compared to absorbance or SDS-PAGE, the eProtein™ Quantification Kit delivers precise, reproducible results across your workflow, and is fully compatible with…
Brochure: Circular eGene™ and Linear eGene™ Comparison
Discover how Nuclera’s eProtein Discovery™ system gives you the freedom to choose between linear and circular eGene™ constructs. Whether you need PCR-based, cost-effective screening with linear eGene™, or ready-to-use, sequence-verified circular eGene™ plasmids for seamless expression in cell-free and E. coli. Compare formats, workflows, and timelines to find the best fit for your protein discovery needs.
