Producing a functional, active ABC transporter, MsbA, in 48 hours

This application note highlights the capabilities of the eProtein Discovery™ system in overcoming the challenges associated with expressing and purifying the membrane protein MsbA, an ABC transporter integral to bacterial lipid transport. By leveraging the system's automated, screening of DNA constructs and customizable cell-free blends, containing membrane mimetics, researchers successfully identified optimal conditions to produce soluble, functional MsbA within 48 hours.

Through this application note, you'll gain insights into:

• The eProtein Discovery™ system's effectiveness in addressing the complexities of membrane protein expression and purification.
• The strategic use of tailored cell-free blends, containing nanodiscs, to enhance protein solubility and activity.
• The streamlined workflow that facilitates rapid progression from DNA construct design to obtaining assay-ready membrane proteins, expediting research and drug development processes.