Start your next protein journey with eProtein Discovery™
Getting proteins shouldn’t be a gamble.
Yet, conventional protein purification workflows still force you through multiple rounds of trial and error. eProtein Discovery™ replaces guesswork with automated, cell-free multiplex screening that ranks the construct/condition pairs most likely to succeed, so you can advance with confidence from the start.
Start here. Identify what works in 24 hours; overnight scale-up delivers µg–mg of assay-ready protein in 48 hours.
Workflow
Step 1: Design
Design and order linear or circular DNA constructs
Step 2: Express & purify
Automated data-generating screens to determine your path to soluble, purifiable proteins
Step 3: Scale up & go!
Scale up proteins to μg and mg scales
Four eProtein Discovery components.
One complete protein solution
Want to learn more about eProtein Discovery?
How does eProtein Discovery work?
In 24 hours, parallel cell-free screens rank the construct, fusion tag, and expression parameters most likely to yield soluble, purified protein. Two dedicated workflows match your targets: Soluble and Membrane. Overnight, scale top conditions off-cartridge to µg–mg so you have assay-ready output by 48 hours.
Intuitive software. Robust decision-grade data. The protein you need to move forward.
Confidently advance with soluble, active, purifiable proteins
Express a wide range of proteins
eProtein Discovery can produce a variety of proteins – from soluble proteins as small as 18kDa to large membrane proteins up to 300kDa: GPCRs, cytokines, growth factors and kinases to name a few.
A data-driven journey to your protein
Automated reports identify the best construct and expression conditions for scale-up: solubility, purifiability, and purified yield.
Produce active protein in 48 hours
DNA-binding dataset for GATA-1, a highly disordered transcription factor. Multiplex screening identified the optimum solubility tag and expression conditions (chaperone and metal cofactor) to produce active GATA-1 (EC50 = 88.09 ng/mL).
Perform structural studies
Ab initio structure showcasing MsbA embedded within an Membrane scaffold protein (MSP)-Lipid based nanodisc. Multiplex screening identified the optimum membrane stabilizer to produce high yields (~200 µg/mL) of MsbA.
What are we hearing?
"The eProtein Discovery System is a welcome new toolkit for membrane protein production. We were commissioned via Imperial Consultants to test the protein quality in our lab – and the early results are promising.”
A protein system like no other
Combining unique cell-free expression systems, novel digital microfluidics, and robust screening data, eProtein Discovery empowers scientists to perform their own protein screening, characterization, and small-scale expression in-house within days. With eProtein Discovery, time is on your side.
Know which proteins have the best chance of success from day one, and take protein projects forward with confidence.
