Resource Library

Join Cube Biotech and Nuclera to discover a rapid, scalable workflow for expressing and stabilizing complex membrane proteins. Learn how Cube Biotech’s membrane mimetics combined with Nuclera’s eProtein Discovery system enable stable, functional, assay-ready membrane proteins in just 48 hours. This webinar highlights case studies on key drug targets, demonstrating how optimized membrane protein production…

Discover how Nuclera’s eProtein Discovery™ system gives you the freedom to choose between linear and circular eGene™ constructs. Whether you need PCR-based, cost-effective screening with linear eGene™, or ready-to-use, sequence-verified circular eGene™ plasmids for seamless expression in cell-free and E. coli. Compare formats, workflows, and timelines to find the best fit for your protein discovery needs.

Achieve accurate, consistent protein quantification at every scale. The same fluorescence-based on-cartridge assay used during screening is now available for scale-up, enabling seamless transition without revalidation or method transfer. With superior sensitivity and specificity compared to absorbance or SDS-PAGE, the eProtein Quantification Kit delivers precise, reproducible results across your workflow, and is fully compatible with…

Get to know more about this platform which gives you soluble, active proteins in 48 hours for your downstream applications.

In this flyer, we outline the 48-hour workflow to deliver protein in-hand including data on reproducibility, quality and quantity.

Struggling with insoluble or inactive proteins? Nuclera’s eProtein Discovery™ Soluble Protein Workflow delivers assay-ready, soluble proteins in just 48 hours. Using AI-guided construct design, high-performing solubility tags, cell-free expression, and automated purification, researchers can screen 24 constructs and 8 conditions simultaneously. With over 1,000 proteins successfully produced across major classes, this end-to-end workflow ensures reproducible…

This application note showcases how Nuclera’s eProtein Discovery™ system, combined with Cytiva’s Biacore™ SPR platform, overcomes major bottlenecks in kinase drug discovery by enabling rapid, high-yield production and kinetic analysis of Bruton’s Tyrosine Kinase (BTK) variants.

This poster illustrates how researchers can rapidly progress from DNA to an expressed, purified, and characterized protein—demonstrated here with Bruton’s tyrosine kinase (BTK)—using the eProtein Discovery and Biacore SPR systems.

This Application Note, covers how we employed the eProtein Discovery System to successfully express and purify full-length Transcription Factor L (TF L), along with a domain of unknown function (L2) and its binding domain (L3).

This application note showcases the eProtein Discovery™ system and the Custom Additive Initiative in addressing DHODH production challenges. By supplementing Cell-free Blends with custom additives such as FMN (a DHODH cofactor, essential for activity) and Brij®-35 (a surfactant known for stabilizing complex membrane proteins), conditions were identified to produce soluble, highly purified, and enzymatically active…