Resource Library

Stop wasting weeks on empirical GPCR detergent screening. This application note demonstrates a rapid, automated cell-free workflow that inserts the β1-adrenergic receptor (β1AR) directly into defined nanodiscs. Using eProtein Discovery™ multiplex screening, researchers can pinpoint optimal lipid environments in 24 hours, scale up to ~100 µg/mL yields, and isolate highly pure, structure-ready GPCRs in less…

Join Dr. Tobias Ost, SVP of Product Development at Nuclera, as we unveil our new, expertly curated eProtein Discovery™ GPCR Nanodiscs. Discover how combining cell-free protein synthesis with co-translational insertion directly into pre-assembled nanodiscs stabilizes GPCRs as they are synthesized. Dr. Ost will demonstrate how the eProtein Discovery™ system automates multiplex screening of GPCR constructs…

Fast-track your route to purified, functional GPCRs with our pre-assembled, quality-controlled nanodisc panel. Designed specifically to target the charge, fluidity, and cholesterol needs for GPCR activity, these validated nanodiscs plug seamlessly into the eProtein Discovery™ multiplex screening and scale-up workflow. See how this tunable design space consistently outperforms generic nanodiscs, ensuring you get high yields…

Give your antibody discovery the early triage step it’s missing with Nuclera Antibody Services. Discover how we triage out non-binders with a 24-hour yes/no binding screen and dedicate SPR to confirmed hits, allowing you to screen 7x more variants for ~$100 per antibody.

Join Dr. Judith Ronau, Senior Scientist at AbbVie, as she details how her team utilizes cell-free protein expression to eliminate the bottlenecks of traditional cell-based production.

Overcome the traditional bottleneck of obtaining stable, functional GPCRs. This poster demonstrates a rapid workflow that bypasses harsh, slow detergent methods by using cell-free protein synthesis (CFPS) to express the β1-adrenergic receptor (β1AR) directly into defined nanodiscs. Using the eProtein Discovery™ system to automate multiplex screening, researchers can identify the optimal lipid environment to deliver…

Cell-free protein synthesis (CFPS) offers a simple, rapid and cost-effective way to express and purify antibodies for binding assessment to triage out non-binders before committing to costly biology.

Unlock GPCRs and other challenging membrane proteins with a cell-free expression workflow that delivers cryo-EM–ready complexes and connects in vitro structures to in vivo function.

Join Cube Biotech and Nuclera to discover a rapid, scalable workflow for expressing and stabilizing complex membrane proteins. Learn how Cube Biotech’s membrane mimetics combined with Nuclera’s eProtein Discovery system enable stable, functional, assay-ready membrane proteins in just 48 hours. This webinar highlights case studies on key drug targets, demonstrating how optimized membrane protein production…

Heme proteins (like phytoglobins) are vital but notoriously hard to express: heme cytotoxicity, folding constraints, and the need to balance redox conditions make protocols slow and brittle—what works for one protein often fails for another. The result is weeks of trial-and-error and low soluble yields. A peer-reviewed study from Lund University shows how cell-free protein…