News: Nuclera launches antibody triage service to advance AI-driven antibody discovery

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Application Note

Application Note: Accelerating BTK Inhibitor Discovery: From DNA to Functional Protein Characterization in Just 5 Days

This application note showcases how Nuclera’s eProtein Discovery™ system, combined with Cytiva’s Biacore™ SPR platform, overcomes major bottlenecks in kinase drug discovery by enabling rapid, high-yield production and kinetic analysis of Bruton’s Tyrosine Kinase (BTK) variants.

Poster

Poster: Screen, Scale, Capture, Characterize: Rapid protein production and characterization of drug-target interactions

This poster illustrates how researchers can rapidly progress from DNA to an expressed, purified, and characterized protein—demonstrated here with Bruton’s tyrosine kinase (BTK)—using the eProtein Discovery and Biacore SPR systems.

Application Note

Application Note: Overcoming Discovery Challenges for Highly Disordered Transcription Factors Using eProtein Discovery™

This Application Note, covers how we employed the eProtein Discovery System to successfully express and purify full-length Transcription Factor L (TF L), along with a domain of unknown function (L2) and its binding domain (L3).

Application Note

Application Note: Producing active, purified human DHODH: Unlocking the power of custom additives on the eProtein Discovery™ system

This application note showcases the eProtein Discovery™ system and the Custom Additive Initiative in addressing DHODH production challenges. By supplementing Cell-free Blends with custom additives such as FMN (a DHODH cofactor, essential for activity) and Brij®-35 (a surfactant known for stabilizing complex membrane proteins), conditions were identified to produce soluble, highly purified, and enzymatically active…

Brochure

Brochure: Visualizing Success: AlphaFold Assisted Protein Variant Design

An overview of how eProtein Discovery™ integrates AlphaFold2 to enable rapid and precise protein variant design. By leveraging 3D structural insights, researchers can identify key features and domains to optimize protein stability, solubility, and function within a streamlined workflow. Whether you're looking to improve your protein engineering strategies or optimize your research workflow, this resource offers valuable insights into automated protein design and expression.

Brochure

Brochure: Functional membrane proteins: Ready in 48 hours

Struggling with membrane protein expression? eProtein Discovery™ uses cell-free protein synthesis and automated additive screening to express and purify high-yield, assay-ready membrane proteins in just 48 hours. Optimize GPCR, ion channel, transporter and membrane-bound enzyme production with a streamlined workflow for cryo-EM, drug discovery, and functional assays.

Application Note

Application Note: Producing a functional, active ABC transporter, MsbA, in 48 hours

This application note highlights the capabilities of the eProtein Discovery™ system in overcoming the challenges associated with expressing and purifying the membrane protein MsbA, an ABC transporter integral to bacterial lipid transport. By leveraging the system’s automated, screening of DNA constructs and customizable cell-free blends, containing membrane mimetics, researchers successfully identified optimal conditions to produce…

Poster

Poster: Functional Membrane Proteins – Ready in 48 Hours

Membrane proteins are the target of 60% of FDA-approved drugs, yet protein extraction and stabilization remain major challenges. eProtein Discovery™ overcomes these hurdles with cell-free protein synthesis, digital microfluidics, and optimized additive screening, delivering functional, correctly folded, and purified membrane proteins in just 48 hours. This poster highlights MsbA (an ABC transporter) and ZMPSTE24 (a…

Poster

Poster: Cell-Free Transcription Factor Production: From Disorder to Data in 48 Hours

Transcription factors are essential regulators of gene expression, yet their intrinsic disorder, zinc‑finger domains, and leucine‑zipper motifs make them notoriously difficult to express and purify with cell‑based methods. Using the cell‑free eProtein Discovery™ platform, combining AI‑guided construct design, automated digital microfluidics, and solubility‑tag screening, Nuclera delivers soluble, full‑length GATA‑1 and c‑Jun. DNA‑binding ELISAs confirmed activity…

Primary Literature

Publication: Accelerating Binding Kinetics with SpyBLI & eProtein Discovery™

Computational design and NGS generate large pools of binders, but purifying each one to measure KD, kon, koff, is slow and costly. A recent peer-reviewed study in RSC Chemical Biology couples Nuclera’s eProtein Discovery™ (cell-free expression) with SpyBLI, a BLI workflow that covalently captures binders via SpyCatcher003–SpyTag003, to deliver robust binding kinetics from crude samples in <24 hours, no purification required. The workflow compresses the clone-express-measure loop to a single, day-scale cycle.