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Webinar

On-demand Webinar: Defying Difficult-to-Express Proteins – Overcoming Protein Expression Challenges

In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours.

Video

Video – Intro to eProtein Discovery™

An overview of eProtein Discovery™, a rapid protein access system located on your benchtop, which enables you to go from DNA to protein in less than 48 hours.

Technical Note

Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression

Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.

Video

Video – How to Carry Out Band-Stab Procedure

Part of the in-the-lab instructional video series

Video

Video – How to Set Up a Run

Part of the in-the-lab instructional video series

Video

Video – Steps to remove bubbles introduced during sample loading

Video

Video – Best Practices for Sample Loading

Part of the in-the-lab instructional video series

Video

Video – How to connect the Base Fluid line

Part of the in the lab instructional video series

Poster

Poster: Enhancing solubility of protein targets: innovations in expression strategies using eProtein Discovery™

An overview of solubility tags and their effects on soluble protein expression, as well as the impact of tag removal, and how it is a necessary and a high-value capability.

Application Note

Application Note: Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery™ System

This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function.

Pages: 1234

eProtein Discovery System

The only end-to-end protein prototyping solution

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