New: Nuclera Expands eProtein Discovery™ with New Membrane Protein Workflow - Read Now

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Technical Note

Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression

Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.

Video

Video – How to Carry Out Band-Stab Procedure

Part of the in-the-lab instructional video series

Video

Video – How to Set Up a Run

Part of the in-the-lab instructional video series

Video

Video – Steps to remove bubbles introduced during sample loading

Video

Video – Best Practices for Sample Loading

Part of the in-the-lab instructional video series

Video

Video – How to connect the Base Fluid line

Part of the in the lab instructional video series

Poster

Poster: Enhancing solubility of protein targets: innovations in expression strategies using eProtein Discovery™

An overview of solubility tags and their effects on soluble protein expression, as well as the impact of tag removal, and how it is a necessary and a high-value capability.

Application Note

Application Note: Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery™ System

This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function.

Application Note

Application Note: Rapid Cell-Free Expression and Purification of B-lymphocyte Antigen CD19 Using eProtein Discovery™

In this Application Note, we leverage the eProtein Discovery™ system to screen 192 expression conditions and optimize the production of soluble CD19 protein within 24 hours. We successfully expressed and purified three CD19 length variants: full-length CD19, the N-terminal extracellular domain, and the C-terminal intracellular domain, overcoming challenges associated with “difficult-to-express” proteins.

Webinar

On-demand Webinar: Predictions to Proteins In-Hand in just 48hrs – Rapidly Express AlphaFold Predictions

The integration of AlphaFold AI within the eProtein Discovery™ framework brings forth a multitude of advantages, facilitating structure guided protein construct design with unprecedented precision.

On-demand webinar Automated Protein Expression Series Prophecy to Proteins In Hand in just 48 hrs - Rapidly Express AlphaFold Predictions Watch Now

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