Resource Library Find supporting resources for our platform. Filter by Resource TypeApplication NoteBrochureFlyerPosterPrimary LiteratureTechnical NoteVideoWebinarWhite Paper Open Filter Webinar Webinar: Accelerating Drug Target Identification and Characterization Screen, Scale, Capture, Characterize – DNA to Protein Characterization in One Week Understanding drug–target interactions is essential to improve leads, optimize pharmacokinetics and predict in vivo efficacy. The process of generating soluble, functional drug targets and studying their interactions with drugs can be complicated and time-consuming, with multiple repeats needed to optimize protein expression and… Technical Note Custom Additives: tailoring protein expression for your research needs Unlock the full potential of the eProtein Discovery™ system with Custom Additives The Custom Additives manual covers how users can customize cell-free blends with additives of their own choice. This increases the flexibility for optimizing protein expression, folding and purification, by using user-chosen additives that are better tailored for their specific protein requirements while maintaining the automation and reproducibility provided by the eProtein Discovery™ system. Application Note Application Note: Producing active, purified human DHODH: Unlocking the power of custom additives on the eProtein Discovery™ system This application note showcases the eProtein Discovery™ system and the Custom Additive Initiative in addressing DHODH production challenges. By supplementing Cell-free Blends with custom additives such as FMN (a DHODH cofactor, essential for activity) and Brij®-35 (a surfactant known for stabilizing complex membrane proteins), conditions were identified to produce soluble, highly purified, and enzymatically active… Application Note Application Note: Overcoming Discovery Challenges for Highly Disordered Transcription Factors Using eProtein Discovery™ This Application Note, covers how we employed the eProtein Discovery System to successfully express and purify full-length Transcription Factor L (TF L), along with a domain of unknown function (L2) and its binding domain (L3). White Paper White Paper: The value delivered by the eProtein Discovery™ workflow This white paper investigates the cost of protein expression and the value delivered by the eProtein Discovery™ workflow. Traditionally, finding suitable conditions to obtain soluble and purifiable recombinant proteins is difficult and time-consuming. Constrained by cost, labor and throughput, typically only a few protein expression conditions are explored, with hit-and-miss results and often requiring multiple… Application Note Application Note: Guiding In Vivo Protein Expression with eProtein Discovery™ CFPS for Disulfide-Rich Proteins The eProtein Discovery System simplifies the transition from cell-free protein synthesis (CFPS) to in vivo. E. coli expression, especially for intricate proteins with disulfide bonds. By evaluating numerous protein variants and up to seven solubility tags, researchers can efficiently identify optimal construct designs. Poster Poster: Screen, Scale, Capture, Characterize: Rapid protein production and characterization of drug-target interactions This poster illustrates how researchers can rapidly progress from DNA to an expressed, purified, and characterized protein—demonstrated here with Bruton’s tyrosine kinase (BTK)—using the eProtein Discovery and Biacore SPR systems. Webinar On-demand Webinar: Defying Difficult-to-Express Proteins – Overcoming Protein Expression Challenges In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours. Video Video – Intro to eProtein Discovery™ An overview of eProtein Discovery™, a rapid protein access system located on your benchtop, which enables you to go from DNA to protein in less than 48 hours. Technical Note Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein. Pages: 123Load More Resources