Resource Library

Join Cube Biotech and Nuclera to discover a rapid, scalable workflow for expressing and stabilizing complex membrane proteins. Learn how Cube Biotech’s membrane mimetics combined with Nuclera’s eProtein Discovery™ system enable stable, functional, assay-ready membrane proteins in just 48 hours. This webinar highlights case studies on key drug targets, demonstrating how optimized membrane protein production…

In this webinar, Dr. Manoj Saxena (Birkbeck, University of London) presents a cell-free, microfluidic multiplex screening workflow that rapidly optimizes soluble transcription factor production. Using this approach, a novel target, Transcription Factor L (TF L) with LIM domains, a homeodomain, and extensive disorder, was produced alongside domain constructs and functionally validated by DNA-binding assays within 48 hours. This workflow moves researchers from disorder to data, enabling assay-ready proteins and accelerating discovery in developmental biology, regenerative medicine, and therapeutic development.

Transcription factors are essential regulators of gene expression, yet their intrinsic disorder, zinc‑finger domains, and leucine‑zipper motifs make them notoriously difficult to express and purify with cell‑based methods. Using the cell‑free eProtein Discovery™ platform, combining AI‑guided construct design, automated digital microfluidics, and solubility‑tag screening, Nuclera delivers soluble, full‑length GATA‑1 and c‑Jun. DNA‑binding ELISAs confirmed activity…

Computational design and NGS generate large pools of binders, but purifying each one to measure KD, kon, koff, is slow and costly. A recent peer-reviewed study in RSC Chemical Biology couples Nuclera’s eProtein Discovery™ (cell-free expression) with SpyBLI, a BLI workflow that covalently captures binders via SpyCatcher003–SpyTag003, to deliver robust binding kinetics from crude samples in <24 hours, no purification required. The workflow compresses the clone-express-measure loop to a single, day-scale cycle.

Struggling with insoluble or inactive proteins? Nuclera’s eProtein Discovery™ Soluble Protein Workflow delivers assay-ready, soluble proteins in just 48 hours. Using AI-guided construct design, high-performing solubility tags, cell-free expression, and automated purification, researchers can screen 24 constructs and 8 conditions simultaneously. With over 1,000 proteins successfully produced across major classes, this end-to-end workflow ensures reproducible…

This application note showcases how Nuclera’s eProtein Discovery™ system, combined with Cytiva’s Biacore™ SPR platform, overcomes major bottlenecks in kinase drug discovery by enabling rapid, high-yield production and kinetic analysis of Bruton’s Tyrosine Kinase (BTK) variants.

This application note highlights the capabilities of the eProtein Discovery™ system in overcoming the challenges associated with expressing and purifying the membrane protein MsbA, an ABC transporter integral to bacterial lipid transport. By leveraging the system’s automated, screening of DNA constructs and customizable cell-free blends, containing membrane mimetics, researchers successfully identified optimal conditions to produce…

Achieve accurate, consistent protein quantification at every scale. The same fluorescence-based on-cartridge assay used during screening is now available for scale-up, enabling seamless transition without revalidation or method transfer. With superior sensitivity and specificity compared to absorbance or SDS-PAGE, the eProtein™ Quantification Kit delivers precise, reproducible results across your workflow, and is fully compatible with…

Discover how Nuclera’s eProtein Discovery™ system gives you the freedom to choose between linear and circular eGene™ constructs. Whether you need PCR-based, cost-effective screening with linear eGene™, or ready-to-use, sequence-verified circular eGene™ plasmids for seamless expression in cell-free and E. coli. Compare formats, workflows, and timelines to find the best fit for your protein discovery needs.

Join experts from IDT, Nuclera, and Aldevron for a webinar showcasing a fully integrated solution that takes you from gene to assay-ready protein in just days — not weeks.